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KMID : 0988420020140010005
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Journal of Dental Science (C.N.U.)
2002 Volume.14 No. 1 p.5 ~ p.22
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Effects of Several Herbal Dentifrices on Incipient Enamel Caries by Surface Hardness Test with Confocal Laser Scanning Microscopy
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Abstract
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The purpose of this study aimed to compare the efficacy of several dentifrices for the promotion of remineralization of subsurface lesion under dynamic condition simulating in vivo carious formation. Subsurface caries lesions were prepared after exposing the extracted, flattened and polished bovine enamel cores to lactate-Carbopol system for 48 hours. After Vickers hardness number(VHN)were determined, the specimens were assigned to 9test groups composed of 12specimens respectively by randomized blocks with VHN stratification. The specimens were sterilized with ethylene oxide gas and exposed to filter-sterilized mixed human saliva for 24hours under continuous stirring or form salivary pellicle. Nine test groups were Akebia quinata dentifrice(Akebia quinata 0.3%), Angelica gigas dentifrice(Angelica gigas 0.3%), Camellia sinensis dentifrice (Crude Catechin 0.4%), negative control dentifrice(only silica), Fluoride-Akebia quinata dentifrice(NaF 0.2%)and positive control dentifrice(DentaQ^(r), NaF 0.2%, Sanguinaria, LG co, Korea). The specimens were daily transferred in freshly inoculated tryptic soy broth by S. sobrinus KCTC 3308. The specimens were provided with 3times a day for 3-minute dentifrice treatment at 4hours interval in the day time between demineralization and remineralization cycles. After the last dentifrices slurry treatment, the specimens were exposed to remineralization/demineralization solution for 6 and 10 hours, respectively. These cycles were repeated for 15days. Then the specimens were cleaned under a stream of tap water and sterilized by exposing to sodium hypochloride solution for 5 minutes followed by distilled water rinse. The VHN were randomly determined in the proximity of the initial areas tested of the same specimens and decoded by treatment groups. The delta VHNs were determined and analyzed by Student t-test and one-way ANOVA. Finally, treated specimens were examined with CLSM for comparing with remineralizaion effect.
The results obtained were from this study were as follows:
1. The minimal inhibitory concentrations(MIC)of the Angelica gigas extract was 3mg/ml, Akebia quinata was 3mg/ml and Camellia sinensis was 4mg/ml on the Streptococcus sobrinus KCTC 3308.
2. The fluoride dentifrices significantly increased surface hardness as compared to the non-fluoride dentifrices(p<0.001).
3. In the non-fluoride dentifrices, Camellia sinensis dentifrice was significantly increased surface hardness as compared to the others(p<0.05).
4. In the fluoride dentifrices, fluoride-Angelica gigas dentifrice was significantly increased surface hardness ascompared to the others(p<0.05).
5. The addition of fluoride decreased the fluorescent lesion depth as compared to the non-flouride dentifrices by CLSM(p<0.05).
6. Statistically significant Pearson correlation coefficients were reverse-correlation between the surface hardness and fluorescent lesion depth by CLSM measured across treatment groups(r=-0.641, p<0.05).
All the presented findings support the conclusions that herbal dentifrice is enough to cariostatic effect if adding in dentifrice and CLSM can be utilized for comparing the efficacy of several dentifrices for the promotion of remineralization of subsurface lesion with surface hardness test.
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